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M9470133.TXT
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1994-07-02
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Document 0133
DOCN M9470133
TI Mechanistic aspects of ascorbate inhibition of human immunodeficiency
virus.
DT 9409
AU Harakeh S; Niedzwiecki A; Jariwalla RJ; Viral Carcinogenesis and
Immunology Program, Linus Pauling; Institute of Science and Medicine,
Palo Alto, CA 94306.
SO Chem Biol Interact. 1994 Jun;91(2-3):207-15. Unique Identifier :
AIDSLINE MED/94251847
AB We have investigated the molecular basis of the inhibitory effect of
ascorbate (vitamin C) on human immunodeficiency virus (HIV) expression
in unstimulated chronically infected and reporter cell lines. Comparison
of intracellular HIV RNA and protein patterns of ascorbate-treated cells
with corresponding patterns of untreated controls, did not show
significant differences in the synthesis or processing of individual
viral RNA and polypeptides, indicating that the inhibitory effect of
ascorbate is not directed at steps of viral transcription or
translation. Enzyme assays on cell extracts showed that the activity of
an HIV LTR-directed reporter protein made in ascorbate-treated cells was
reduced to approximately 11% relative to that of untreated control.
These results, combined with previous observations on the suppression of
HIV RT activity, are consistent with a mechanism of action in which
ascorbate exerts a posttranslational inhibitory effect on HIV by causing
impairment of enzymatic activity.
DE beta-Galactosidase/BIOSYNTHESIS Ascorbic Acid/*PHARMACOLOGY Blotting,
Northern Cell Line Cell Survival/DRUG EFFECTS Genes, Reporter Human
HIV/*DRUG EFFECTS/GENETICS/METABOLISM HIV Long Terminal Repeat/DRUG
EFFECTS Reverse Transcriptase/ANTAGONISTS & INHIB RNA,
Viral/BIOSYNTHESIS Transcription, Genetic/DRUG EFFECTS Translation,
Genetic/DRUG EFFECTS Viral Proteins/BIOSYNTHESIS JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).